glucose concentration in culture medium affects mrna expression of trpv1 and cb1 receptors and changes capsaicin toxicity in pc12 cells
نویسندگان
چکیده
objective (s):hyperglycemia is widely recognized as the underlying cause for some debilitating conditions in diabetic patients. the role of cannabinoid cb1 and vanilloid trpv1 receptors and their endogenous agonists, endovanilloids, in diabetic neuropathy is shown in many studies. here we have used pc12 cell line to investigate the possible influence of glucose concentration in culture medium on cytoprotective or toxic effects of a cb1 [win55 212-2 (win)], or trpv1 [capsaicin (cas)] agonist. materials and methods: cell viability was tested using the mtt assay. we have also measured trpv1 and cb1 transcripts by real time reverse transcription-polymerase chain reaction while cells were grown in low (5.5 mm) and high (50 mm) glucose concentrations. results:real time pcr results indicated that high glucose medium increased (p<0.01) trpv1 mrna and decreased (p <0.001) that of cb1. cell culture tests show that hyperglycemic cells are more vulnerable (dose × medium, f (3,63)=41.5, p<0.001) to the toxic effects of capsaicin compared to those grown in low glucose medium. conclusion: these findings propose that hyperglycemic conditions may result in neuronal cell death because of inducing a counterbalance between cytotoxic trpv1 and cytoprotective cb1 receptors.
منابع مشابه
Glucose concentration in culture medium affects mRNA expression of TRPV1 and CB1 receptors and changes capsaicin toxicity in PC12 cells
Objective (s):Hyperglycemia is widely recognized as the underlying cause for some debilitating conditions in diabetic patients. The role of cannabinoid CB1 and vanilloid TRPV1 receptors and their endogenous agonists, endovanilloids, in diabetic neuropathy is shown in many studies. Here we have used PC12 cell line to investigate the possible influence of glucose concentration in culture medium o...
متن کاملGlucose concentration in culture medium affects mRNA expression of TRPV1 and CB1 receptors and changes capsaicin toxicity in PC12 cells
OBJECTIVE S Hyperglycemia is widely recognized as the underlying cause for some debilitating conditions in diabetic patients. The role of cannabinoid CB1 and vanilloid TRPV1 receptors and their endogenous agonists, endovanilloids, in diabetic neuropathy is shown in many studies. Here we have used PC12 cell line to investigate the possible influence of glucose concentration in culture medium on ...
متن کامل: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes
هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...
The role of locus coeruleus nucleus TRPV1 receptors in the development and expression of morphine dependence
Objective(s): This study investigated the role of locus coeruleus (LC) nucleus TRPV1 receptors (TRPV1r) in the expression and development of morphine physical dependence by intra-LC administration of AMG9810 (selective TRPV1r antagonist) in male Wistar rats. Materials and Methods: For assessing the development of morphine dependence, AMG9810 (0.0...
متن کاملsynthesis of sulfides from alcohols and thiols in solvent-freeconditions and deoxygenation of sulfoxides
کاتالیست یک سنتز جدید برای تیواترها توصیف شده است. واکنش الکل ها با آریل، هتروآریل و آلکیل تیو ل ها درحضور 1،3،5- تری آزو- 2،4،6- تری فسفرین-2،2،4،4،6،6 هگزاکلراید ((tapc به عنوان یک کاتالیست موُثر، بازده های خوب تا عالی از تیواترها را حاصل می کند. علاوه براین، واکنش تحت شرایط بدون فلز و بدون حلال پیش می رود، بنابراین یک مکمل جالب برای روش های شناخته شده سنتز تیواترها ارائه می دهد. یک مکانیسم ا...
15 صفحه اولمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
iranian journal of basic medical sciencesجلد ۱۷، شماره ۹، صفحات ۶۷۳-۶۷۸
کلمات کلیدی
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023